DNA, RNA hybridization kits.(New Products)

Bioscience Technology;  January 1, 2004 ;     160 words ......complete kit includes all reagents needed for in situ hybridization including the anti-biotin or anti-fluorescein antibody, biotinylated secondary antibody, enzyme-streptavidin conjugate, chromogen/substrate, counterstain, mounting medium, protein block, and all...

Western Blotting method.(March's Technology)

Bioscience Technology;  March 1, 2005 ;     166 words ......probed by secondary antibodies without risk of non-specific binding. The method minimizes strong non-specific binding of biotinylated secondary antibodies to some urinary proteins. Millipore Corp., 800-645-5476, www.millipore.com For information specific to this...

Bead-based assay kits.(Immunological Reagents And Assays Focus)

Bioscience Technology;  June 1, 2004 ;     206 words ......phosphorylated serine, threonine, or tyrosine residues detect phosphorylation sites. Primary antibodies are detected using a biotinylated secondary anti-mouse antibody. Secondary antibodies are delecled in turn using streptavidin conjugated to highly fluorescent phycoerythrin...

Bead-based Kinase assays.(Cell Culture/Cell Analysis)

Bioscience Technology;  May 1, 2004 ;     187 words ......specifically recognize phosphorylated serinc, threonine, or tyrosine residues. Primary antibodies are detected using a biotinylated secondary anti-mouse antibody. Secondary antibodies are detected in turn using streptavidin conjugated to highly fluorescent phycoerythrin...

Rosenthal fibers and eosinophilic granular bodies in a classic acoustic...

Archives of Pathology & Laboratory Medicine;  November 1, 1997 ;     700+ words ......Leu-7 (Beckton-Dickinson, San Jose, Calif), using a biotinylated secondary antibody and an avidin-biotin complex detection system...to GFAP (Dako Corp, Carpinteria, Calif), also using a biotinylated secondary antibody and an avidin-biotin complex detection system...

A quantum leap for fluorescence

The Scientist;  February 24, 2003 ;     391 words ......biotin-tagged molecules. In a recent paper, researchers used such conjugates to visualize Her2 and cytoskeletal components via biotinylated secondary antibodies.1 Though customers are currently limited to a streptavidin coating, Watson says the company will soon release...

Extracorporeal irradiated autogenous osteochondral graft: A HISTOLOGICAL...

Journal of Bone and Joint Surgery;  July 1, 2005 ;     700+ words ......DakoCytomation Co, Ltd (Kyoto, Japan). The primary antibodies were applied at a dilution of 1:1000. Following incubation with biotinylated secondary antibody, avidin-enzyme complex was applied. Antibody binding was visualised by incubating the slides in 3',3-diaminobenzidine...

p53 protein alterations in adult astrocytic tumors and oligodendrogliomas

Neurology India;  April 1, 2004 ;     700+ words ......100) followed by overnight incubation at 4C. This was followed by incubation for 30 minutes at room temperature with biotinylated secondary antibody of anti-mouse immunoglobulins. Subsequently, peroxidase conjugated streptavidin was applied and sections were...

Repair of human articular cartilage after implantation of autologous...

Journal of Bone and Joint Surgery;  November 1, 1999 ;     700+ words ......epitopes, as described previously.10,11 Sections incubated with monoclonal antibodies were subsequently labelled with a biotinylated secondary antibody, the endogenous peroxidase activity being blocked with 0.3% hydrogen peroxide in methanol before labelling...

Cytokeratin 7 and cytokeratin 20 in primary urinary bladder carcinoma and...

Archives of Pathology & Laboratory Medicine;  July 1, 2001 ;     700+ words ......dilution; Dako Corporation, Carpinteria, Calif) and CK20 (1:50 dilution; Dako) were incubated overnight at 4C, followed by biotinylated secondary antibody and peroxidase-labeled streptavidin. Three-aminoethylcarbazole was used as chromogen in the presence of hydrogen...

Apoptosis in microciona prolifera allografts.

The Biological Bulletin;  October 1, 2003 ;     700+ words ......slides were then washed with phosphate-buffered saline (PBS, pH 7.5) (Sigma), followed by treatment with appropriate biotinylated secondary antibody (Vector Laboratories). After another wash in PBS, the slides were treated with avidin-biotinylated enzyme complex...

Training alone, not the tripeptide RGD, modulates calexcitin in...

The Biological Bulletin;  October 1, 2003 ;     700+ words ......Calexcitin was immunolabeled with a primary polyclonal antibody (25U2; cloned from squid optic lobe and raised in rabbit; 1:1000 dil) (2, 3); subsequently, color was developed with a biotinylated secondary antibody, and avidin-bound microper

Basaloid squamous cell carcinoma of the larynx and hypopharynx

The Annals of Otology, Rhinology & Laryngology;  December 1, 1997 ;     700+ words ......PBS) washes (pH 7.6) prior to immunostaining. Overnight incubation with primary antibodies was performed in all cases. Biotinylated secondary antibodies and avidin-biotinylated horseradish peroxidase complex (Vector ABC Elite Kit) were obtained from Vector Laboratories...

The localization of Fos B, a member of transcription factor AP-1 family,...

Folia Histochemica et Cytobiologica;  January 1, 2004 ;     700+ words ......rabbit polyclonal antibody against Fos B, the sections were incubated in the primary antibody for 2 h and then in the biotinylated secondary antibody for 30 min. Negative controls were performed by substituting the primary antibody with nonimmune rabbit serum...